Determination of Para-Phenylenediamine (PPD) in Henna Samples Collected from Libyan Local Markets Using HPLC

Henna is frequently used as body adornment in Libya, the Arabic world, and some African Asian countries. It part of traditions cultures. The addition para-phenylenediamine (PPD) to natural henna increases risk allergic contact dermatitis well toxic effects, which sometimes lead severe toxicity death. Cases have already been reported Al-Bayda city - Libya. This study aimed identify presence determination PPD by High-Performance Liquid Chromatography (HPLC). In current study, eleven samples were collected from local markets A rapid, simple, reliable method developed validated for using 50% methanol solution solvent. over a wide linear range 5 – 25 µg/ml with correlation coefficients being consistently greater than 0.997. minimum level was observed random sample mixed (paste2) (0.0104 % w/w), while Royal Black showed highest content (11.9107 w/w). HPLC measurements indicated that results concentrations Red Shikha (natural henna) free (ND) (Not Detected). higher samples. red analyzed this below allowable limits set Scientific Committee on Consumer Products (SCCP) US Food Drugs Administration. While it recommended limit.